PhytophthoraV1, Main, Exploration, bibRecord, 000569

Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System.

Identifieur interne : 000569 ( Main/Exploration ); précédent : 000568; suivant : 000570

Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System.

Auteurs : Jia Wu [République populaire de Chine] ; Hafiz Muhammad Khalid Abbas [République populaire de Chine] ; Jiale Li [République populaire de Chine] ; Yuan Yuan [République populaire de Chine] ; Yunjun Liu [République populaire de Chine] ; Guoying Wang [République populaire de Chine] ; And Wubei Dong [République populaire de Chine]

Source :

Biomolecules [ 2218-273X ] ; 2019.

RBID : pubmed:31878275

Descripteurs français

KwdFr :
- Bacillus subtilis (génétique), Biotechnologie (MeSH), Expression des gènes (MeSH), Isatis (génétique), Membrane cellulaire (effets des médicaments et des substances chimiques), Peptides (génétique), Peptides (isolement et purification), Peptides (pharmacologie), Peptides antimicrobiens cationiques (génétique), Peptides antimicrobiens cationiques (isolement et purification), Peptides antimicrobiens cationiques (pharmacologie), Tabac (microbiologie).
MESH :
- effets des médicaments et des substances chimiques : Membrane cellulaire.
- génétique : Bacillus subtilis, Isatis, Peptides, Peptides antimicrobiens cationiques.
- isolement et purification : Peptides, Peptides antimicrobiens cationiques.
- microbiologie : Tabac.
- pharmacologie : Peptides, Peptides antimicrobiens cationiques.
- Biotechnologie, Expression des gènes.

English descriptors

KwdEn :
- Antimicrobial Cationic Peptides (genetics), Antimicrobial Cationic Peptides (isolation & purification), Antimicrobial Cationic Peptides (pharmacology), Bacillus subtilis (genetics), Biotechnology (MeSH), Cell Membrane (drug effects), Gene Expression (MeSH), Isatis (genetics), Peptides (genetics), Peptides (isolation & purification), Peptides (pharmacology), Tobacco (microbiology).
MESH :
- chemical , genetics : Antimicrobial Cationic Peptides, Peptides.
- chemical , isolation & purification : Antimicrobial Cationic Peptides, Peptides.
- chemical , pharmacology : Antimicrobial Cationic Peptides, Peptides.
- drug effects : Cell Membrane.
- genetics : Bacillus subtilis, Isatis.
- microbiology : Tobacco.
- Biotechnology, Gene Expression.

Abstract

The situation of drug resistance has become more complicated due to the scarcity of plant resistance genes, and overcoming this challenge is imperative. Isatis indigotica has been used for the treatment of wounds, viral infections, and inflammation for centuries. Antimicrobial peptides (AMPs) are found in all classes of life ranging from prokaryotes to eukaryotes. To identify AMPs, I. indigotica was explored using a novel, sensitive, and high-throughput Bacillus subtilis screening system. We found that IiR515 and IiR915 exhibited significant antimicrobial activities against a variety of bacterial (Xanthomonas oryzae, Ralstonia solanacearum, Clavibacter michiganensis, and C. fangii) and fungal (Phytophthora capsici and Botrytis cinerea) pathogens. Scanning electron microscope and cytometric analysis revealed the possible mechanism of these peptides, which was to target and disrupt the bacterial cell membrane. This model was also supported by membrane fluidity and electrical potential analyses. Hemolytic activity assays revealed that these peptides may act as a potential source for clinical medicine development. In conclusion, the plant-derived novel AMPs IiR515 and IiR915 are effective biocontrol agents and can be used as raw materials in the drug discovery field.

DOI: 10.3390/biom10010030
PubMed: 31878275
PubMed Central: PMC7023251

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000314
to stream Main, to step Curation: 000314

Le document en format XML

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<term>Antimicrobial Cationic Peptides (isolation & purification)</term>
<term>Antimicrobial Cationic Peptides (pharmacology)</term>
<term>Bacillus subtilis (genetics)</term>
<term>Biotechnology (MeSH)</term>
<term>Cell Membrane (drug effects)</term>
<term>Gene Expression (MeSH)</term>
<term>Isatis (genetics)</term>
<term>Peptides (genetics)</term>
<term>Peptides (isolation & purification)</term>
<term>Peptides (pharmacology)</term>
<term>Tobacco (microbiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Bacillus subtilis (génétique)</term>
<term>Biotechnologie (MeSH)</term>
<term>Expression des gènes (MeSH)</term>
<term>Isatis (génétique)</term>
<term>Membrane cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Peptides (génétique)</term>
<term>Peptides (isolement et purification)</term>
<term>Peptides (pharmacologie)</term>
<term>Peptides antimicrobiens cationiques (génétique)</term>
<term>Peptides antimicrobiens cationiques (isolement et purification)</term>
<term>Peptides antimicrobiens cationiques (pharmacologie)</term>
<term>Tabac (microbiologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antimicrobial Cationic Peptides</term>
<term>Peptides</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antimicrobial Cationic Peptides</term>
<term>Peptides</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antimicrobial Cationic Peptides</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Membrane</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus subtilis</term>
<term>Isatis</term>
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<term>Peptides</term>
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<front><div type="abstract" xml:lang="en">The situation of drug resistance has become more complicated due to the scarcity of plant resistance genes, and overcoming this challenge is imperative. Isatis indigotica has been used for the treatment of wounds, viral infections, and inflammation for centuries. Antimicrobial peptides (AMPs) are found in all classes of life ranging from prokaryotes to eukaryotes. To identify AMPs, I. indigotica was explored using a novel, sensitive, and high-throughput Bacillus subtilis screening system. We found that IiR515 and IiR915 exhibited significant antimicrobial activities against a variety of bacterial (Xanthomonas oryzae, Ralstonia solanacearum, Clavibacter michiganensis, and C. fangii) and fungal (Phytophthora capsici and Botrytis cinerea) pathogens. Scanning electron microscope and cytometric analysis revealed the possible mechanism of these peptides, which was to target and disrupt the bacterial cell membrane. This model was also supported by membrane fluidity and electrical potential analyses. Hemolytic activity assays revealed that these peptides may act as a potential source for clinical medicine development. In conclusion, the plant-derived novel AMPs IiR515 and IiR915 are effective biocontrol agents and can be used as raw materials in the drug discovery field.</div>
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 Fortune Isolated by a Bacillus subtilis Expression System.</ArticleTitle>
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<Abstract><AbstractText>The situation of drug resistance has become more complicated due to the scarcity of plant resistance genes, and overcoming this challenge is imperative. Isatis indigotica has been used for the treatment of wounds, viral infections, and inflammation for centuries. Antimicrobial peptides (AMPs) are found in all classes of life ranging from prokaryotes to eukaryotes. To identify AMPs, I. indigotica was explored using a novel, sensitive, and high-throughput Bacillus subtilis screening system. We found that IiR515 and IiR915 exhibited significant antimicrobial activities against a variety of bacterial (Xanthomonas oryzae, Ralstonia solanacearum, Clavibacter michiganensis, and C. fangii) and fungal (Phytophthora capsici and Botrytis cinerea) pathogens. Scanning electron microscope and cytometric analysis revealed the possible mechanism of these peptides, which was to target and disrupt the bacterial cell membrane. This model was also supported by membrane fluidity and electrical potential analyses. Hemolytic activity assays revealed that these peptides may act as a potential source for clinical medicine development. In conclusion, the plant-derived novel AMPs IiR515 and IiR915 are effective biocontrol agents and can be used as raw materials in the drug discovery field.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName>
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<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei Province, China.</Affiliation>
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<AffiliationInfo><Affiliation>Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</Affiliation>
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<AffiliationInfo><Affiliation>Guangdong Key Laboratory for New Technology Research of Vegetables, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</Affiliation>
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<Initials>G</Initials>
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<Author ValidYN="Y"><LastName>Dong</LastName>
<ForeName>And Wubei</ForeName>
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<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei Province, China.</Affiliation>
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Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System.

Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System.

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